ISSCR 2019: 26-29 JUNE

LOS ANGELES

(F-2029) 

EFFECT OF SMALL MOLECULE INDUCED BEIGE ADIPOCYTES ON CARDIOMYOCYTES AGAINST HYPOXIA/REOXYGENATION

Kim, Sang Woo - Institute for Bio-Medical Convergence,Catholic Kwandong University, Incheon, Korea

Kang, Misun - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Lee, Jiyun - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Park, Jun-Hee - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Song, Byeong-Wook - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Choi, Jung-Won - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Lim, Soyeon - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Kim, Il-Kwon - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Lee, Seahyoung - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Hwang, KI-Chul - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Beige adipocytes gained much attention as an alternative cellular target in regulating metabolic homeostasis. The development of the beige cells that has beneficial metabolic, but how thermogenic stimuli activate and control beige adipocytes is not fully understood. Here, we investigated that the effects and mechanism of isoliquiritigenin (ILG) on adipose-derived stem cells (ASCs) differentiate into functional beige/brown adipocytes. We found that ASCs derived white adipocytes are able to switch to a brown phenotype by expressing both UCP1 and CIDEA according to low dose ILG treatments. Although various pharmacological activities of ILG, the possible role of ILG in white-to beige transdifferentiation of adipose-derived stem cells have never been explored. Moreover, we demonstrated that ILG small molecule dose-response in distinct regulatory mechanisms and functions to induce the white-to-beige transdifferentiation of adipose-derived stem cells. Then, we sought to determine if small molecule induced beige adipocytes into impaired heart tissue affected surround fat in the heart. Therefore, we investigated the expression levels of proteins associated with oxidation, inflammation, and death signals in cardiomyocytes with beige cells under hypoxia/reoxygenation conditions for their roles in many physiological processes in the heart. These studies suggest that the induction of thermogenic adipocytes might offer a new approach to combating human metabolic disorders and ischemic heart diseases.

Funding Source: This study was funded by NRF-2018R1A2B6008629, 2016R1D1A1B03935124, and NRF-2015M3A9E6029519.