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EFFECT OF CONNEXIN 43 AND SGSM3 THROUGH HIF-1Α-MEDIATED MODULATION IN RAT BONE MARROW-DERIVED MESENCHYMAL STEM CELLS
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  • 2019-07-02 16:14:52

ISSCR 2019: 26-29 JUNE

LOS ANGELES

(F-2030)  

 

EFFECT OF CONNEXIN 43 AND SGSM3 THROUGH HIF-1Α-MEDIATED MODULATION IN RAT BONE MARROW-DERIVED MESENCHYMAL STEM CELLS

 

Jung, Seung Eun - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Kang, Misun - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Lee, Jiyun - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Park, Jun-Hee - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Song, Byeong-Wook - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Choi, Jung-Won - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Lim, Soyeon - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Kim, Sang Woo - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Kim, Il-Kwon - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Lee, Seahyoung - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea

Hwang, Ki-Chul - Institute for Bio-Medical Convergence, Catholic Kwandong University, Incheon, Korea


Connexin 43 (Cx43) contributes gap junction-mediated communication as a gap junction protein but has shown channel independent functions. Many reports have suggested that Cx43 regulates other cellular mechanisms, including cell cycles, differentiation, and proliferation. Recent evidence suggests that connexins, and in particular Cx43, may have additional effects that may be important in cell death and survival by mechanisms independent of cell to cell communication. In the previous study, we found that Cx43-interaction protein, small G protein signaling modulator 3 (SGSM3) plays a critical role in stress cells. Moreover, their interaction plays a key role in Cx43 internalization for connexin turnover in cardiomyocytes of infarcted hearts. Here, we investigated for SGSM3, a potential partner of Cx43, in an attempt to identify for enhancing survival markers in bone marrow-derived mesenchymal stem cells (MSCs). Cx43 co-immunoprecipitated analysis identifying two proteins, and gap junction proteins were predicted that SGSM3 was highly correlated with Cx43 in GeneMANIA network analysis. Results of Hif1a and Sgsm3 siRNA knockdown experiments suggest that SGSM3 possibly plays a role in the cellular response to stress or ischemia with Cx43 dependently on Hif1α. In conclusion, these data demonstrate a role for SGSM3 in Cx43 endocytic trafficking and further substantiate its role in Cx43 turnover. This knowledge of SGSM3-mediated regulation of Cx43 may help to identify a novel therapeutic target to counteract the loss of Cx43 or impairment of Cx43-GJIC that disrupt normal cell functions and are associated with many human diseases.


Funding Source: This study was funded by 2018R1D1A1B07049416 and NRF-2015M3A9E6029519

이전글 EFFECT OF SMALL MOLECULE INDUCED BEIGE ADIPOCYTES ON CARDIOMYOCYTES AGAINST HYPOXIA/REOXYGENATION
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